Qualitative tests of functional groups in Carbohydrate

 Qualitative tests of functional groups in Protein 

Aim:-   Detection of  Carbohydrates 

Preparation of Sample solution for Carbohydrate test. 

Make 0.2% and 2% solutions of the Dextrose-D or Fructose and perform the following experiments in order to identify the reducing action of glucose and fructose :

Preliminary test for identification of unknown substance from the sample

Sr. No.	Test	Observation	Inference
1	Preliminary Test	coagulation	Protein  may be present carbohydrates may be present
		No coagulation	carbohydrates may be present
2	Biurate test Take 3 ml  of 5% O.S. in a test tube, add 1 ml of 40% NaOH solution to make it strongly alkaline, and then add 2 drops of 1 % copper sulphate solution.	Violet or pink colour appears.	Protein may be present.
3		No violet or pink colour	Carbohydrates may be present.