FUNDAMENTALS OF BIOCHEMISTRY and ANIMAL PHYSIOLOGY: CONTROLLING AND COORDINATING SYSTEMS
1. Qualitative tests of functional groups in carbohydrates, proteins and lipids.
2. Estimation of protein by colorimetric method.
3. Estimation of carbohydrates by colorimetric method.
4. Demonstration of paper chromatography of amino acids.
5. Action of amylase or papain enzyme under optimum conditions.
6. Effect of pH, temperature, and inhibitors on the action of amylase.
7. Demonstration of protein separation by SDS-PAGE.
8. Recording of simple muscle twitch/ Cardiogram – demonstration. (Virtual frog/ computer generated) (Analysis of given graph of Frog- muscle twitch or cardiogram In the examination students are provided with any one computer-generated graph and supposed to ‘Analyze the given graph and explain details of principle, procedure, result, Inference, and viva-voce based on the given practical is expected)
9. Study of permanent slides (T.S./V,S.) - of Mammalian organs using permanent slides:
i) Tooth
ii) Salivary gland
iii) Stomach
iv) IIeum v) Liver
vi) Pancreas
vii) Kidney
viii) Testis
ix) Ovary
10. Study of ABO blood group system and blood group antigens
11. Microtomy:
- Study of principle
- Procedure and mechanism of micro-technique and microtome
- Flow chart of technique
- study of procedure and observation of HE staining technique/ whole mount using permanent slides (study of protocol using flowchart).
12. Study visit: Visit to wetlands, medical college, pathology laboratory and blood bank
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