STUDY OF RAT SPERM AND VAGINAL SMEAR

 

STUDY  OF RAT SPERM AND VAGINAL, Blood group 

 

 SPERM : 

Each sperm has a head middle piece and a long tail. The head contains a dark staining nucleus with the less dense tip that caused the acrosome. The middle piece contains internally a spirally coiled thread. In tail is long and the sperm moues with a lashing movement using the tail the immature.  Sperm, when leaving the testies, are nonmotile they have no power of movements they which into the epididymis they become mature the fluid secreted by the glands nourishes them and makes them viable the glandular secretion is sufficiently declined so that it can neutralize the acidity of the vagina fluid.

 

Vaginal smear :

  The vagina lies ventral to the rectum but dorsal to the urethra the vagina thus straight backword and opens to the exterior by the vaginal genital aperture the valve the opening of the urethra the vagina and urethra together form a short common passage called valva

 

 

Study of  blood group Antigen

Aim : To classify the blood groups

Chemicals and Reagent: 70% alcohols blood group antisera A.B.D.

Equipment : Pricking needles glass slide microscopetc.

Principle : 

Detection of blood group depends upon antigen-antibody interaction Antigen antibody Antigen- B, when a particular antigen is present and corres ponding the antibody is absent when particular it will react reacted with respective anti body it will react with an antigen present on the R.B. forming a complex of the clump the clumping is also called clotting or Agglutination.

 

Procedure :

 1) Keeping all sterile condition take three drops of blood on a clean glass side.

2) Add the antiserum A.D.B. to each drop of blood.

3) Shake the drop of blood gently with the help of tooth pins needle and observe the clumpiny.

4) Interpret the result at follows.

 Result :

  For studying the classification of blood group.

I performed the expect or blood group checking hence my own blood group is ‘O’ Negative.