Study of procedure and observation of HE staining technique protocol using flowchart
Protocol for H&E staining:
Slide
↓
Dewax
in
Xylene (10- 15 Min. )
↓
(Hydration through Alcoholic grades )
100%
alcohol (10- 15 Min. )
↓
90%
alcohol
↓
70%
alcohol
↓
50%
alcohol
↓
30%
alcohol
↓
Distilled
water
↓
Haematoxylin
↓
Distilled
water
↓
Dehydration
↓
30%
alcohol
↓
50%
alcohol
↓
70%
alcohol
↓
90%
alcohol
↓
Alcoholic eosin (prepared in 90 % alcohol)
↓
90%
alcohol
↓
100%
alcohol
↓
Xylene
↓
Mount
in D.P.X
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